Imaging NEUROPICT
In brief
Our platform enables multi-scale imaging from the cell to the whole organism in support of Neuroscience research.
The users are offered training, assistance and advice. We also collaborate on scientific and technology projects.
The platform currently has nine acquisition systems and one image analysis workstation:
- 1 light-sheet microscope (Miltenyi)
- 2 multiphoton microscopes (Leica SP8 and Femtonics Femto2D Dual)
- 3 confocal laser scanning microscopes (Zeiss LSM880 Airyscan, LSM700 and Leica SP8)
- 3 microscopes with structured illumination (Apotomes2, Zeiss)
- 1 epifluorenscence microscope with a 4-slide scanner (Axioimager M2, Zeiss)
- 1 image analysis workstation with IMARIS software.
New users are trained to become autonomous and are assisted by the technical staff.
Booking
Booking via the GIPSI website is mandatory. New users please contact Sandrine Guyon : sandrine.guyon[At]universite-paris-saclay.fr
Pricing
Hours of use are billed quarterly.
| NeuroPSI members | Others academics | |
| Confocal and bi-photon | 15 € | 25 € |
| Airyscan confocal | 20 € | 30 € |
| Lightsheet | 20 € | 30 € |
| Structured illumination | 2 or 4€ (motorised) | 10 € |
| Imaris workstation | 2 € | 10 € |
Operations manager
Sandrine GUYON – Research Engineer, CNRS
Aurélie HEUZE – Engineer, CNRS
Scientific referent
Julien BOUVIER – Researcher, CNRS
UltraMicroscope II, Miltenyi Biotec
Objectives :
- 1.1X objective lens, 0.1 NA Working distance: 15.7 mm (H2O) / 16.0 mm (Clarity/Scale) / 17.6 mm (DBE)
Corrected for: 450-850 nm - 4X objective lens, 0.3 NA Working distance: 5.6-6.0 mm
Lasers and filter sets :
- 488 Laser ET525/50m Ø32 mm emission filter
- 561 Laser ET590/33m Ø32 mm emission filter
- 639Laser ET680/30m
Camera : Andor Zyla 5.5 sCMOS Camera
Microscope confocal Zeiss LSM880 Airyscan
Objectives :
- Plan apochromat 10x/0,45 (air)
- Plan apochromat 20x/0,8 (air)
- Plan apochromat 40x/1,3 (oil) with DIC
Lasers :
- 458nm, 488nm, 514nm, 561nm et 633nm
Scanners :
- 3PMTs (mode spectral) + Airy scan 32 GaAsp-PMT
Microscope biphoton Leica SP8
Objectives :
- 25x HC Fluotar L, 0.95 NA, immersion : water ; working distance 2,5
Lasers :
- 405nm, 448nm, 488nm, 552nm
- Coherent Chameleon Vision II
Filter sets :
- BP 585/40, BP 525/50, SP680 ; Dichroïc BS 560.
Scanners :
- 2 PMTs (spectral detection) and 2 PMTsT-RLD (Green/Red).
Microscope biphoton Femtonics, Femto2D Dual – – (Description in progress)
Objectives :20x et 4x
Lasers :
Filters sets :
Scanner résonnant et détecteurs externes non déscannés PMT:
Microscope confocal Leica TCS SP8 AOBS – DM6000CS
Objectives :
- 10x Plan APO, 0,4 NA ; immersion: dry ; working distance 2,2
- 25x IRAPO, 0,95 NA ; immersion: water ; working distance 2,5
- 40x Plan APO, 1,1 NA ; immersion: water ; working distance 0,65
Lasers :
- Ar, 65 mW: 458, 476, 488, 496, 514 nm
- HeNe, 2 mW: 594 nm
- HeNe, 10 mW : 633 nm
- DPSS, 20 mW: 561 nm
- UV : Diode, 50mW : 405 nm.
Microscope confocal Zeiss LSM 700
Objectives :
- 10x EC Plan Néofluar, 0.3 NA, immersion: dry ; working distance 5,2
- 20x EC Plan Néofluar, 0.5 NA, immersion: dry ; working distance 2,0
- 40x EC Plan Néofluar, 1.3 NA, immersion: oil ; working distance 0,21
- 63x Plan Apochromat , 1.4 NA, immersion: oil ; working distance 0,19
Lasers :
- 405nm, 5mW
- 488nm, 10mW
- 555nm, 10mW
- 639nm, 5mW
Filters sets :
- 49 DAPI shift free, EX G 365, BS FT 395, EM BP 445/50
- 38 Endow GFP shift free, EX BP 470/40, BS FT 495, EM BP 525/50
- 20 Rhodamin shift free, EX BP 546/12, BS FT 560, EM BP 575-640
3 microscopes with structured illumination (Apotome 2 Zeiss)
1 epifluorescence microscope with a 4 slides scanner (AxioImager M2 Zeiss)
1 image analyzis worksation
- PC Dell PRECISION 7920
- 2x processors Xeon Gold 6248R (24 Coeurs, 3.0 – 4.0 Ghz, 35.75 Mo cache)
- 1 To RAM
- GPU NVIDIA RTX A60000 (48 Go RAM, 10752 coeurs CUDA)
- 32″ monitor.
Softwares :
- IMARIS for Neuroscientists avec extensions « Stitcher » et « Cell », version 9.9
- ZEN lite
- LasX
- Fiji
- DeepLabCut.
