Group leader: Martial Ruat

Signal Transduction & Developmental Neuropharmacology

In brief

Our group aims at deciphering the molecular and pharmacological mechanisms implicated in adult neural stem cells (NSCs) maintenance. We develop animal models to study NSCs and their progeny and chemical screening to identify novel innovative therapeutic strategies for brain repair and for treating brain tumors.

Research context and strategy

• In the adult brain, neural stem cells (NSCs) represent a reservoir of cells that could be potentially recruited to stimulate glio- and neurogenesis, and may therefore have a broad range of therapeutic applications for brain repair. Aberrant activation of signaling pathways in NSCs or in neural progenitors have been associated to brain tumors.

• We are using and developing conditional mouse models for studying adult NSCs and their progeny in the main neurogenic niches of the brain. We are focusing our work towards understanding the functional roles of the Hedgehog signaling pathway proposed to modulate the maintenance of adult neurogenic niches and to regulate the proliferation and differentiation of neuronal and glial precursors. We are currently analyzing modifications of this pathway such as those linked to human mutations responsible for the development of brain tumors such as medulloblastoma. The pharmacological treatment of these disturbances is investigated using synthetic molecules that we develop with organic and medicinal chemists.

• Thus, we have characterized and patented reference agonists and antagonists of the Smoothened receptor which mediates most of the effects of the Hedgehog signals. These molecules have potential interests in the treatment of demyelinating diseases and brain cancers. They should contribute to elucidate mechanisms regulating the maintenance of precursor cells that represent a major challenge for the discovery of effective treatments for neurodegenerative diseases and cancers.

Selected publications

  • Alice Del Giovane, Mariagiovanna Russo, Linda Tirou, Helene Faure, Martial Ruat, Sonia Balestri, Carola Sposato, Francesco Basoli, Alberto Rainer, Abdelmoumen Kassoussi, Elisabeth Traiffort, Antonella Ragnini-Wilson. Smoothened/AMPK signaling in oligodendroglial cell maturation. Frontiers in Cellular Neuroscience, (2022) In press. (DOI: 10.3389/fncel.2021.801704)
  • Linda Tirou, Mariagiovanna Russo, Hélène Faure, Giuliana Pellegrino, Clément Demongin, Mathieu Daynac, Ariane Sharif, Jeremy Amosse, Soazig Le Lay, Raphaël Denis, Serge Luquet, Mohammed Taouis, Yacir Benomar, Martial Ruat. Sonic Hedgehog receptor Patched deficiency in astrocytes enhances glucose metabolism in mice. Molecular Metabolism, 2021, 47:101172. (DOI: 10.1016/j.molmet.2021.101172)
  • Linda Tirou, Mariagiovanna Russo, Hélène Faure, Giuliana Pellegrino, Ariane Sharif, Martial Ruat. C9C5 positive mature oligodendrocytes are a source of Sonic Hedgehog in the mouse brain. PloS one, 2020, 15 (2), pp e0229362, (DOI: 10.1371/journal.pone.0229362)
  • Chia-Hsiang Chang, Marco Zanini, Hamasseh Shirvani, Jia-Shing Cheng, Hua Yu, Chih-Hsin Feng, Audrey L. Mercier, Shiue-Yu Hung, Antoine Forget, Chun-Hung Wang, Sara Maria Cigna, I. Ling Lu, Wei-Yi Chen, Sophie Leboucher, Won-Jing Wang, Martial Ruat, Nathalie Spassky, Jin-Wu Tsai, Olivier Ayrault. Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation. Developmental Cell, 2019, 48 (2), pp 184-199.e185, (DOI: 10.1016/j.devcel.2018.12.017
  • Alexandra Chicheportiche, Martial Ruat, François D. Boussin, Mathieu Daynac. Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System. Cellular Quiescence: Methods and Protocols 2018. New York, NY: Springer New York. p 69-78. (DOI: 10.1007/978-1-4939-7371-2_5)
  • Mathieu Daynac, Linda Tirou, Hélène Faure, Marc-André Mouthon, Laurent R. Gauthier, Heidi Hahn, François D. Boussin, Martial Ruat. Hedgehog Controls Quiescence and Activation of Neural Stem Cells in the Adult Ventricular-Subventricular Zone. Stem cell reports, 2016, 7 (4), pp 735-748, (DOI: 10.1016/j.stemcr.2016.08.016)
  • Audrey Fleury, Lucile Hoch, M. Carmen Martinez, Hélène Faure, Maurizio Taddei, Elena Petricci, Fabrizio Manetti, Nicolas Girard, André Mann, Caroline Jacques, Jérôme Larghero, Martial Ruat, Ramaroson Andriantsitohaina, Soazig Le Lay. Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway. Scientific Reports, 2016, 6 (1), pp 23479, (DOI: 10.1038/srep23479)
  • Lucile Hoch, Hélène Faure, Hermine Roudaut, Angele Schoenfelder, Andre Mann, Nicolas Girard, Laure Bihannic, Olivier Ayrault, Elena Petricci, Maurizio Taddei, Didier Rognan, Martial Ruat. MRT-92 inhibits Hedgehog signaling by blocking overlapping binding sites in the transmembrane domain of the Smoothened receptor. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015, 29 (5), pp 1817-1829, (DOI: 10.1096/fj.14-267849)
  • Martial Ruat. Top Med Chem: The Smoothened Receptor in Cancer and Regenerative Medicine. 2015. Springer International Publishing. 1-210 p. (DOI: 10.1007/978-3-319-19755-5)
  • Julien Ferent, Loic Cochard, Hélène Faure, Maurizio Taddei, Heidi Hahn, Martial Ruat, Elisabeth Traiffort. Genetic activation of Hedgehog signaling unbalances the rate of neural stem cell renewal by increasing symmetric divisions. Stem cell reports, 2014, 3 (2), pp 312-323, (DOI: 10.1016/j.stemcr.2014.05.016)

Contrats obtenus par l’équipe et partenariat avec des entreprises pharmaceutiques depuis 2004

  • 2004 GIS-maladies rares. Subvention (Subv.) (Collaboration).
  • 2005-2006, FRC. Subv.
  • 2005 ARSEP. Subv.
  • 2006-2007 ARC . Subv.
  • 2006 Ligue contre le Cancer. Subv.
  • 2006-2008 INCA “Cancer Détection d’Innovations 2006”. Subv. (Collaboration)
  • 2007-2008 AFM. Subv. – 2007-2010 ANR. Subv.
  • 2008-2010 ANR. Subv. – 2004-2006, Société BIOGEN (bourse CIFRE).
  • 2004 Société SERVIER, Etude du mode d’action du strontium ranelate.
  • 2007-2008 LEO Pharma Research.

Enseignements

Martial RUAT :
Depuis 2012 : L2 de biologie, Université Paris Sud Orsay (3h)

  • 2012 : 6th Summer School Medicinal Chemistry. Faculty of Chemistry and Pharmacy at the University of Regensburg. 26-28 September (Lecture, 1h).
  • 2008-2011 : Master of Drug Design and Synthesis : Siena university, Siena, Italy, “New therapeutic strategy : from allosteric modulators to medication” (6h)
  • 2005-2011 : M2, Physiologie Cellulaire,Biothérapies et Innovations Thérapeutiques – “Voie de signalisation Hedgehog. Implications thérapeutiques ” : Chatenay-Malabry (2h30)
2021

• Alice Del Giovane, Mariagiovanna Russo, Linda Tirou, Helene Faure, Martial Ruat, Sonia Balestri, Carola Sposato, Francesco Basoli, Alberto Rainer, Abdelmoumen Kassoussi, Elisabeth Traiffort, Antonella Ragnini-Wilson. Smoothened/AMPK signaling in oligodendroglial cell maturation. Frontiers in Cellular Neuroscience, (2022) In press. (DOI: 10.3389/fncel.2021.801704)

• Linda Tirou, Mariagiovanna Russo, Hélène Faure, Giuliana Pellegrino, Clément Demongin, Mathieu Daynac, Ariane Sharif, Jeremy Amosse, Soazig Le Lay, Raphaël Denis, Serge Luquet, Mohammed Taouis, Yacir Benomar, Martial Ruat (2021) Sonic Hedgehog receptor Patched deficiency in astrocytes enhances glucose metabolism in mice. Molecular Metabolism, 47:101172. doi: 10.1016/j.molmet.2021.101172

2020

• Tirou L, Russo M, Faure H, Pellegrino G, Sharif A, and Ruat M, (2020) C9C5 positive mature oligodendrocytes are a source of Sonic Hedgehog in the mouse brain. PLOS ONE, 15(2): p. e0229362. doi: 10.1371/journal.pone.0229362

2019

• Chang CH, Zanini M, Shirvani H, Cheng JS, Yu H, Feng CH, Mercier AL, Hung SY, Forget A, Wang CH, Cigna SM, Lu IL, Chen WY, Leboucher S, Wang WJ, Ruat M, Spassky N, Tsai JW, and Ayrault O, (2019) Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation. Dev Cell, 48(2): p. 184-199 e5. doi: 10.1016/j.devcel.2018.12.017

2018

• Chicheportiche A, Ruat M, Boussin FD, and Daynac M, (2018) Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System. Methods Mol Biol, 1686: p. 69-78. doi: 10.1007/978-1-4939-7371-2_5

2016

• Daynac M, Tirou L, Faure H, Mouthon MA, Gauthier LR, Hahn H, Boussin FD, and Ruat M. (2016) Hedgehog Controls Quiescence and Activation of Neural Stem Cells in the Adult Ventricular-Subventricular Zone. Stem Cell Reports. doi: 10.1016/j.stemcr.2016.08.016

• Manetti F, Petricci E, Gabrielli A, Mann A, Faure H, Gorojankina T, Brasseur L, Hoch L, Ruat M, and Taddei M. (2016) Design, synthesis and biological characterization of a new class of osteogenic (1H)-quinolone derivatives. Eur J Med Chem 121, 747-757. doi: 10.1016/j.ejmech.2016.05.062

• Kiefer L, Beaumard F, Gorojankina T, Faure H, Ruat M, and Dodd RH. (2016) Design and synthesis of calindol derivatives as potent and selective calcium sensing receptor agonists. Bioorg Med Chem 24 : 4, 554-569. doi: 10.1016/j.bmc.2015.12.019

• Chiarenza A, Manetti F, Petricci E, Ruat M, Naldini A, Taddei M, and Carraro F. (2016) Novel Acylguanidine Derivatives Targeting Smoothened Induce Antiproliferative and Pro-Apoptotic Effects in Chronic Myeloid Leukemia Cells. PLoS One 11 : 3, e0149919. doi: 10.1371/journal.pone.0149919

• Fleury A, Hoch L, Martinez MC, Faure H, Taddei M, Petricci E, Manetti F, Girard N, Mann A, Jacques C, Larghero J, Ruat M, Andriantsitohaina R, and Le Lay S. (2016) Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway. Sci Rep 6, 23479. doi: 10.1038/srep23479

2015

• Daynac, M., L. Morizur, T. Kortulewski, L. R. Gauthier, M. Ruat, M.-A. Mouthon and F. Boussin, D. (2015). “Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics.” J Vis Exp(103) : e53247. doi: 10.3791/53247

• Gache, Y., F. Brellier, S. Rouanet, S. Al-Qaraghuli, M. Goncalves-Maia, E. Burty-Valin, S. Barnay, S. Scarzello, M. Ruat, N. Sevenet, M. F. Avril and T. Magnaldo (2015). “Basal Cell Carcinoma in Gorlin’s Patients : a Matter of Fibroblasts-Led Protumoral Microenvironment ?” PLoS One 10(12) : e0145369. doi: 10.1371/journal.pone.0145369
• Hoch L, Faure H, Roudaut H, Schoenfelder A, Mann A, Girard N, Bihannic L, Ayrault O, Petricci E, Taddei M, Rognan D, and Ruat M. (2015) MRT-92 inhibits Hedgehog signaling by blocking overlapping binding sites in the transmembrane domain of the Smoothened receptor. Faseb J, 29 : 5, 1817-1829. doi: 10.1096/fj.14-267849

• Ruat M, Faure H, and Daynac M. (2015) Smoothened, Stem Cell Maintenance and Brain Diseases. In. Topics in Medicinal Chemistry, Springer Berlin Heidelberg.
• Ruat M, and Hoch L. (2015) Smoothened Inhibitors in Cancer. In. Topics in Medicinal Chemistry, Springer Berlin Heidelberg

2014

• Ferent J, Cochard L, Faure H, Taddei M, Hahn H, Ruat M, and Traiffort E. (2014) Genetic activation of Hedgehog signaling unbalances the rate of neural stem cell renewal by increasing symmetric divisions. Stem Cell Report 3 2, 312–323. doi: 10.1016/j.stemcr.2014.05.016

• Ferent J, Ruat M, and Traiffort E. (2014) [Symmetric or asymmetric division : sonic Hedgehog controls the fate of neural stem cells]. Med Sci (Paris) 30 : 6-7, 705-708. doi: 10.1051/medsci/20143006026

• Ruat M, Hoch L, Faure H, and Rognan D. (2014) Targeting of Smoothened for therapeutic gain. Trends Pharmacol Sci 35 : 5, 237-246. doi: 10.1016/j.tips.2014.03.002

2013

• Ferent J, Ruat M, and Traiffort E. (2013) Investigation of the proteolipid protein promoter activity during demyelination and repair. Differentiation 85 : 4-5, 182-189. doi: 10.1016/j.diff.2013.05.002

• Gorojankina T, Hoch L, Faure H, Roudaut H, Traiffort E, Schoenfelder A, Girard N, Mann A, Manetti F, Solinas A, Petricci E, Taddei M, Ruat M. (2013) Discovery, molecular and pharmacological characterization of GSA-10, a novel small molecule positive modulator of Smoothened. Mol Pharmacol 83 : 5, 1020-1029. doi: 10.1124/mol.112.084590

• Ruat M, and Traiffort E. (2013) Roles of the calcium sensing receptor in the central nervous system. Best Pract Res Clin Endocrinol Metab 27 : 3, 429-442. doi: 10.1016/j.beem.2013.03.001

• Traiffort E, O’Regan S, and Ruat M. (2012) The choline transporter-like family SLC44 : Properties and roles in human diseases. Mol Asp Med, 34 : 2-3, 646-654. doi: 10.1016/j.mam.2012.10.011

• Ferent J, Zimmer C, Durbec P, Ruat M, and Traiffort E. (2013) Sonic Hedgehog signaling is a positive oligodendrocyte regulator during demyelination. J Neurosci, 33 : 5, 1759-1772. doi: 10.1523/JNEUROSCI.3334-12.2013

• Renault MA, Chapouly C, Yao Q, Larrieu-Lahargue F, Vandierdonck S, Reynaud A, Petit M, Jaspard-Vinassa B, Belloc I, Traiffort E, Ruat M, Duplàa C, Couffinhal T, Desgranges C, Gadeau AP. (2013) Desert hedgehog promotes ischemia-induced angiogenesis by ensuring peripheral nerve survival. Circ Res 112 : 5, 762-770. doi: 10.1161/CIRCRESAHA.113.300871

• Ferent J, Ruat M, and Traiffort E. (2013) Investigation of the proteolipid protein promoter activity during demyelination and repair. Differentiation 85 : 4-5, 182-189. doi: 10.1016/j.diff.2013.05.002

• Ruat M, Hoch L, Faure H, and Rognan D. (2013) [Structure of the Smoothened receptor]. Med Sci (Paris) 29 : 10, 855-860. doi: 10.1051/medsci/20132910012

• Renault MA, Robbesyn F, Chapouly C, Yao Q, Vandierdonck S, Reynaud A, Belloc I, Traiffort E, Ruat M, Desgranges C, and Gadeau AP. (2013) Hedgehog-Dependent Regulation of Angiogenesis and Myogenesis Is Impaired in Aged Mice. Arterioscler Thromb Vasc Biol, 33(12):2858-66. doi: 10.1161/ATVBAHA.113.302494

2012

• Horn A, Palumbo K, Cordazzo C, Dees C, Akhmetshina A, Tomcik M, Zerr P, Avouac J, Gusinde J, Zwerina J, Roudaut H, Traiffort E, Ruat M, Distler O, Schett G, and Distler JH. (2012) Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis. Arthritis Rheum 64 : 8, 2724-2733. doi: 10.1002/art.34444

• Ruat M, Roudaut H, Ferent J, Traiffort E (2011) Hedgehog trafficking, cilia and brain functions. Differentiation 83(2):S97-104. doi: 10.1016/j.diff.2011.11.011

• Solinas A, Faure H, Roudaut H, Traiffort E, Schoenfelder A, Mann A, Manetti F, Taddei M, and Ruat M. (2012) Acylthiourea, Acylurea and Acylguanidine Derivatives with potent Hedgehog inhibiting Activity. J Med Chem 55 : 4, 1559-1571. doi: 10.1021/jm2013369

• Horn A, Kireva T, Palumbo-Zerr K, Dees C, Tomcik M, Cordazzo C, Zerr P, Akhmetshina A, Ruat M, Distler O, Beyer C, Schett G, and Distler JH. (2012) Inhibition of hedgehog signalling prevents experimental fibrosis and induces regression of established fibrosis. Ann Rheum Dis 71 : 5, 785-789. doi: 10.1136/annrheumdis-2011-200883

• Loupy A, Ramakrishnan SK, Wootla B, Chambrey R, de la Faille R, Bourgeois S, Bruneval P, Mandet C, Christensen EI, Faure H, Cheval L, Laghmani K, Collet C, Eladari D, Dodd RH, Ruat M, and Houillier P. (2012) PTH-independent regulation of blood calcium concentration by the calcium-sensing receptor. J Clin Invest 122 : 9, 3355-3367 doi: 10.1172/JCI57407

2011

• Liu T, Wang Y, Chen X, Sun L, Guo N, Zheng H, Zheng L, Ruat M, Han W, Zhang CX, Zhou Z (2011) An inhibitory effect of extracellular ca on ca-dependent exocytosis. PloS One 6 e24573. doi: 10.1371/journal.pone.0024573

• Roudaut H, Traiffort E, Gorojankina T, Vincent L, Faure H, Schoenfelder A, Mann A, Manetti F, Solinas A, Taddei M, Ruat M (2011) Identification and mechanism of action of the acylguanidine MRT-83, a novel potent Smoothened antagonist. Mol Pharmacol. 79(3):453-60. doi: 10.1124/mol.110.069708

• Bidet M, Joubert O, Lacombe B, Ciantar M, Nehmé R, Mollat P, Brétillon L, Faure H, Bittman R, Ruat M, Mus-Veteau I (2011) The hedgehog receptor patched is involved in cholesterol transport. PLoS One. 6:e23834. doi: 10.1371/journal.pone.0023834

• Guerlet G, Spangenberg T, Mann A, Faure H, Ruat M (2011) Synthesis and biological evaluation of desmethylveramiline, a micromolar Hedgehog inhibitor. Bioorg Med Chem Lett. 21(12):3608-12. doi: 10.1016/j.bmcl.2011.04.103

• Ruat M, Angot E, Traiffort E (2011). Shh signal and its functional roles in normal and diseased brain. Med Sci (Paris). Nov ;27(11):979-85. doi: 10.1051/medsci/20112711014

2010

• Kiefer L, Gorojankina T, Dauban P, Faure H, Ruat M & Dodd RH (2010) Design and synthesis of cyclic sulfonamides and sulfamates as new calcium sensing receptor agonists. Bioorg Med Chem Lett : 20(24):7483-7. doi: 10.1016/j.bmcl.2010.10.006

• Manetti F, Faure H, Roudaut H, Gorojankina T, Traiffort E, Schoenfelder A, Mann A, Solinas A, Taddei M & Ruat M (2010) Virtual screening-based discovery and mechanistic characterization of the acylthiourea MRT-10 family as smoothened antagonists. Mol Pharmacol 78 : 658-665. doi: 10.1124/mol.110.065102

• Chen W, Bergsman JB, Wang X, Gilkey G, Pierpoint CR, Daniel EA, Awumey EM, Dauban P, Dodd RH, Ruat M & Smith SM (2010) Presynaptic external calcium signaling involves the calcium-sensing receptor in neocortical nerve terminals. PLoS One 5 : e8563. doi: 10.1371/journal.pone.0008563

• Traiffort E, Angot E & Ruat M (2010) Sonic Hedgehog signaling in the mammalian brain. J Neurochem 113 : 576-590. doi: 10.1111/j.1471-4159.2010.06642.x

2009

• Faure H, Gorojankina T, Rice N, Dauban P, Dodd RH, Brauner-Osborne H, Rognan D & Ruat M (2009) Molecular determinants of non-competitive antagonist binding to the mouse GPRC6A receptor. Cell Calcium 46 : 323-332..
• Fromigue O, Hay E, Barbara A, Petrel C, Traiffort E, Ruat M & Marie PJ (2009) Calcium sensing receptor-dependent and receptor-independent activation of osteoblast replication and survival by strontium ranelate. J Cell Mol Med 13 : 2189-2199..
• Joubert O., Nehmé R., Fleury D., De Rivoyre M., Bidet M., Polidori A., Ruat M., Pucci B., Mollat P., Mus-Veteau I., (2009) « Functional studies of membrane-bound and purified human Hedgehog receptor Patched expressed in yeast. », Biochimica et Biophysica Acta / Biochim biophysica Acta [Amsterdam], 1788 (9) : 1813-21

2008

• Angot E., Loulier K., Nguyen-Ba-Charvet K. T., Gadeau A.-P., Ruat M., Traiffort E., (2008) “Chemoattractive activity of sonic hedgehog in the adult subventricular zone modulates the number of neural precursors reaching the olfactory bulb.”, Stem Cells, 26(9) : 2311-20.
• Harno E., Edwards G., R. Geraghty A., T. Ward D., Faure H., Ruat M., H. Weston A., H. Dodd R., Dauban P., (2008) “Evidence for the presence of GPRC6A receptors in rat mesenteric arteries.”, Cell Calcium, 44(2) : 210-9.
• Weston A. H., Absi M., Harno E., Geraghty A. R., Ward D. T., Ruat M., Dodd R., Dauban P., Edwards G., (2008) “The expression and function of Ca(2+)-sensing receptors in rat mesenteric artery ; comparative studies using a model of type II diabetes.”, British Journal of Pharmacology, 154(3) : 652-62

2007

• Collin L., Doretto S., Malerba M., Ruat M., Borrelli E., (2007) “Oligodendrocyte ablation affects the coordinated interaction between granule and Purkinje neurons during cerebellum development.”, Experimental Cell Research, 313(13) : 2946-57.
• Masdeu C., Bernard V., Faure H., Traiffort E., Ruat M., (2007) “Distribution of Smoothened at hippocampal mossy fiber synapses.”, Neuroreport, 18(4) : 395-9

2006

• Angot E., Loulier K., Traiffort E., Ruat M., (2006) “Analysis of cell proliferation in the mouse brain upon the adenovirus-mediated transfer of sonic hedgehog and hedgehog interacting protein”, International Journal of Developmental Neuroscience, 24(8) : 496-7.
• Fromigue O., Barbara A., Hay E., Petrel C., Traiffort E., Ruat M., Marie P.J., (2006) “Strontium ranelate stimulates murine osteoblast replication independently of calcium sensing receptor-mediated ERK1/2 activation”, Osteoporosis International, 17(Suppl. 2) : S351-2.
• Kessler A., Faure H., Petrel C., Rognan D., Césario M., Ruat M., Dauban P., H. Dodd R., (2006) “N1-Benzoyl-N2-[1-(1-naphthyl)ethyl]-trans-1,2-diaminocyclohexanes : Development of 4-Chlorophenylcarboxamide (Calhex 231) as a New Calcium Sensing Receptor Ligand Demonstrating Potent Calcilytic Activity”, Journal of Medicinal Chemistry, 49(17) : 5119 -28 [hal].
• Loulier K., Ruat M., Traiffort E., (2006) “Increase of proliferating oligodendroglial progenitors in the adult mouse brain upon Sonic hedgehog delivery in the lateral ventricle”, Journal of Neurochemistry, 98(2) : 530-42.
• Martinez M.C., Larbret F., Zobairi F., Coulombe J., Debili N., Vainchenker W., Ruat M., Freyssinet J.-M., (2006) “Transfer of differentiation signal by membrane microvesicles harboring hedgehog morphogens.”, Blood, 108(9) : 3012-20.
• Masdeu C., Faure H., Coulombe J., Schoenfelder A., Mann A., Brabet I., Pin J.-P., Traiffort E., Ruat M., (2006) “Identification and characterization of Hedgehog modulator properties after functional coupling of Smoothened to G15.”, Biochemical and Biophysical Research Communications, 349(2) : 471-9

2005 and before.

• Loulier K., Ruat M., Traiffort E., (2005) “Analysis of hedgehog interacting protein in the brain and its expression in nitric oxide synthase-positive cells.”, Neuroreport, 16(17) : 1959-62.
• Pascual O., Traiffort E., P. Baker D., Galdes A., Ruat M., Champagnat J., (2005) “Sonic hedgehog signalling in neurons of adult ventrolateral nucleus tractus solitarius.”, European Journal of Neuroscience, 22 : 389-96.
• Ray K., Tisdale J., Dodd R., Dauban P., Ruat M., Northup J., (2005) “Calindol, a Positive Allosteric Modulator of the Human Ca2+ Receptor, Activates an Extracellular Ligand-binding Domain-deleted Rhodopsin-like Seven-transmembrane Structure in the Absence of Ca2+”, Journal of Biological Chemistry, 280(44) : 37013-20.
• Rossel M., Loulier K., Feuillet C., Alonso S., Carroll P., (2005) “Reelin signaling is necessary for specific step in the migration of hindbrain efferent neurons”, Development, 132(6) : 1175-85.
• Silve C., Petrel C., Leroy C., Bruel H., Mallet E., Rognan D., Ruat M., (2005) “Delineating a Ca2+ Binding Pocket within the Venus Flytrap Module of the Human Calcium-sensing Receptor”, Journal of Biological Chemistry, 280(45) : 37917-23.
• Silve C., Petrel C., Leroy C., Mallet E., Bruel H., Rognan D., Ruat M., (2005) “Identification of a novel activating mutation in the calcium sensing receptor gene supports the physiological relevance of the Venus flytrap module.”, Journal of Bone and Mineral Research, 20(9) Suppl.1 : S182.
• Traiffort E., Ruat M., O’Regan S., Meunier F. M., (2005) “Molecular characterization of the family of choline transporter-like proteins and their splice variants”, Journal of Neurochemistry, 92(5) : 1116-25 [hal].
• Weston A. H., Absi M., Ward D. T., Ohanian J., Dodd R. H., Dauban P., Petrel C., Ruat M., Edwards G., (2005) “Evidence in favor of a calcium-sensing receptor in arterial endothelial cells : studies with calindol and Calhex 231”, Circulation Research, 97(4) : 391-8.
• Coulombe J., Traiffort E., Loulier K., Faure H., Ruat M., (2004) “Hedgehog interacting protein in the mature brain : membrane-associated and soluble forms.”, Molecular and Cellular Neuroscience, 25(2) : 323-33.
• Coulombe J., Faure H., Robin B., Ruat M., (2004) “In vitro effects of strontium ranelate on the extracellular calcium-sensing receptor.”, Biochemical and Biophysical Research Communications / Biochemistry and Biophysics Research Communications, 323(4) : 1184-90.
• Couvé-Privat S., Le Bret M., Traiffort E., Queille S., Coulombe J., Bouadjar B., Avril M. F., Ruat M., Sarasin A., Daya-Grosjean L., (2004) “Functional analysis of novel sonic hedgehog gene mutations identified in basal cell carcinomas from xeroderma pigmentosum patients.”, Cancer Research, 64(10) : 3559-65.
• Kessler A., Faure H., Roussanne M. C., Ferry S., Ruat M., Dauban P., Dodd R. H., (2004) “N(1)-Arylsulfonyl-N(2)-(1-(1-naphthyl)ethyl)-1,2-diaminocyclohexanes : a new class of calcilytic agents acting at the calcium-sensing receptor.”, ChemBioChem, 5(8) : 1131-6.
• Kessler A., Faure H., Petrel C., Ruat M., Dauban P., Dodd R. H., (2004) “N2-benzyl-N1-(1-(1-naphthyl)ethyl)-3-phenylpropane-1,2-diamines and conformationally restrained indole analogues : development of calindol as a new calcimimetic acting at the calcium sensing receptor.”, Bioorg Med Chem Lett, 14(12) : 3345-49.
• Loulier K., Coulombe J., Traiffort E., Faure H., Ruat M., (2004) “Molecular and functional characterization of the Hedgehog signalling pathway in the adult brain”, Gene Therapy, 11 : S163-4.
• Petrel C., Kessler A., Dauban P., Dodd R. H., Rognan D., Ruat M., (2004) “Positive and negative allosteric modulators of the Ca2+-sensing receptor interact within overlapping but not identical binding sites in the transmembrane domain.”, The Journal of Biological Chemistry, 279(18) : 18990-7.
• Ruat M., Petrel C., (2004) “Comment moduler l’activité du récepteur aux ions calcium extracellulaires ? [New insights into the pharmacology of the extracellular calcium sensing receptor]”, Médecine sciences : M/S, 20(11) : 980-5.
• Traiffort E., Dubourg C., Faure H., Rognan D., Odent S., Durou M.-R., David V., Ruat M., (2004) “Functional characterization of sonic hedgehog mutations associated with holoprosencephaly.”, The Journal of Biological Chemistry, 279(41) : 42889-97.
• Petrel C., Kessler A., Maslah F., Dauban P., Dodd R. H., Rognan D., Ruat M., (2003) “Modeling and mutagenesis of the binding site of Calhex 231, a novel negative allosteric modulator of the extracellular Ca(2+)-sensing receptor.”, The Journal of Biological Chemistry, 278(49) : 49487-94.
• Ruat M., (2003) “Pharmacologie du récepteur aux ions calcium extracellulaires. [Pharmacology of the extracellular calcium ion receptor]”, Thérapie, 58(5) : 395-401.
• Charytoniuk D., Traiffort E., Hantraye P., Hermel J.-M., Galdes A., Ruat M., (2002) “Intrastriatal sonic hedgehog injection increases Patched transcript levels in the adult rat subventricular zone.”, European Journal of Neuroscience, 16(12) : 2351-7.
• Charytoniuk D., Porcel B., Rodríguez Gomez J., Faure H., Ruat M., Traiffort E., (2002) “Sonic Hedgehog signalling in the developing and adult brain.”, Journal of Physiology-Paris, 96(1-2) : 9-16.
• Coulombe J., Faure H., Robin B., Tsouderos Y., Ruat M., (2002) “Activation of the rat and mouse cation-sensing receptor by strontium ranelate and its modulation by extracellular calcium.”, Osteoporosis International, 13(suppl.1) : S25.
• Faure H., Ruat M., Kessler A., Dauban P., Dodd R.H., (2002) “Pharmacological characterization of antagonists acting at the extracellular calcium sensing receptor.”, Osteoporosis International, 13(suppl.1) : S29.
• Hack I., Bancila M., Loulier K., Carroll P., Cremer H., (2002) “Reelin is a detachment signal in tangential chain-migration during postnatal neurogenesis.”, Nature Neuroscience, 5(10) : 939-45.
• Salès N., Hässig R., Rodolfo K., Di Giamberardino L., Traiffort E., Ruat M., Frétier P., Moya K. L., (2002) “Developmental expression of the cellular prion protein in elongating axons.”, European Journal of Neuroscience, 15(7) : 1163-77.
• Trottier S., Chotard C., Traiffort E., Unmehopa U., Fisser B., Swaab D.F., Schwartz J.C., (2002) “Co-localization of histamine with GABA but not with galanin in the human tuberomamillary nucleus.”, Brain Research, 939(1-2) : 52-64.
• Traiffort E., Moya K. L., Faure H., Hässig R., Ruat M., (2001) “High expression and anterograde axonal transport of aminoterminal sonic hedgehog in the adult hamster brain.”, European Journal of Neuroscience, 14(5) : 839-50.
• Charytoniuk D., Traiffort E., Pinard E., Issertial O., Seylaz J., Ruat M., (2000) “Distribution of bone morphogenetic protein and bone morphogenetic protein receptor transcripts in the rodent nervous system and up-regulation of bone morphogenetic protein receptor type II in hippocampal dentate gyrus in a rat model of global cerebral ischemia.”, Neuroscience, 100(1) : 33-43.
• Dauban P., Ferry S., Faure H., Ruat M., Dodd R. H., (2000) “N1-Arylsulfonyl-N2-(1-aryl)ethyl-3-phenylpropane-1,2-diamines as novel calcimimetics acting on the calcium sensing receptor.”, Bioorganic ; Medicinal Chemistry Letters, 10(17) : 2001-4.
• Ferry S., Traiffort E., Stinnakre J., Ruat M., (2000) “Developmental and adult expression of rat calcium-sensing receptor transcripts in neurons and oligodendrocytes.”, European Journal of Neuroscience, 12(3) : 872-84.
• Le Bouffant F., Le Minter P., Traiffort E., Ruat M., Sladeczek F., (2000) “Multiple subcellular localizations of PCTAIRE-1 in brain.”, Molecular and Cellular Neuroscience, 16(4) : 388-95.
• Morisset S., Traiffort E., Arrang J.M., Schwartz J.C., (2000) “Changes in histamine H3 receptor responsiveness in mouse brain.”, Journal of Neurochemistry, 74(1), 339-46.
• O’Regan S., Traiffort E., Ruat M., Cha N., Compaore D., Meunier F.-M., (2000) “An electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins.”, Proceedings of the National Academy of Sciences of the United States of America, 97(4) : 1835-40.
• Morrisset S., Sahm U.G., Traiffort E., Tardivel-Lacombe J., Arrang J.M., Schwartz J.C., (1999) “Atypical neuroleptics enhance histamine turnover in brain via 5-hydroxytryptamine(2A) receptor blockade.”, Journal of Pharmacology and Experimental Therapeutics, 288(2) : 590-6.
• Traiffort E., Charytoniuk D., Watroba L., Faure H., Sales N., Ruat M., (1999) “Discrete localizations of hedgehog signalling components in the developing and adult rat nervous system.”, European Journal of Neuroscience, 11(9) : 3199-214.
• Traiffort E., Charytoniuk D., Faure H., Ruat M., (1998) “Regional distribution of Sonic Hedgehog, patched, and smoothened mRNA in the adult rat brain.”, Journal of Neurochemistry, 70(3) : 1327-30.