Équipe Mohammed Taouis

Neuroendocrinologie moléculaire de la prise alimentaire

En bref

NMPA étudie les mécanismes impliqués dans l’installation de la leptino- et insulino-résistances au niveau hypothalamique. Notre objectif est l’étude de l’influence à long terme de l’environnement périnatal (endocrinien et métabolique) sur le contrôle hypothalamique de l’homéostasie énergétique. As previously described, hypothalamic leptin sensitivity is critical in the control of energy homeostasis and neuronal organization. Previously, we have demonstrated that leptin, insulin, CNTF and serotonin share common signaling pathways. We have showed that both leptin and insulin resistances are associated, linking then obesity to type 2 diabetes through most likely resistin. This part of our project will be organized in the following tasks.

Notre recherche

Projets scientifiques
1. Molecular and cellular mechanisms involved in hypothalamic insulin and leptin resistance
* Cellular and molecular mechanisms of resistin/TLR4-dependent hypothalamic nuclei inflammation and insulin resistance
Resistin induces hypothalamic neuro-inflammation (Benomar et al., 2013)

2. Impact of hypothalamic hormonal and metabolic environment during perinatal periods
* Expression profile of hypothalamic microRNA :
According to our previous studies we hypothesize that changes of specific miRNA expression levels may be involved, at least partially, in the process of diet or hormonal induced alteration of hypothalamic neuronal organization during early life. Furthermore, we will continue the improvement this technique by developing a guideline to quantify barcoding impact in multiplex microRNA expression profiles using Illumina technology.

Validation and the physiological relevance of miRNAs.
The characterization of specific miRNAs will be validated by in situ hybridization (ISH) to specifically confirm their localization in specific hypothalamic nuclei.

Validation and the physiological relevance of hypothalamic miRNAs.

Publications Choisies

> Benomar Y, Taouis M. Molecular Mechanisms Underlying Obesity-Induced Hypothalamic Inflammation and Insulin Resistance: Pivotal Role of Resistin/TLR4 Pathways. Front Endocrinol (Lausanne). 2019 Mar 8;10:140. doi: 10.3389/fendo.2019.00140. eCollection 2019. Review.PMID: 30906281

> Le Stunff H, Véret J, Kassis N, Denom J, Meneyrol K, Paul JL, Cruciani-Guglielmacci C, Magnan C, Janel N. Deciphering the Link Between Hyperhomocysteinemia and Ceramide Metabolism in Alzheimer-Type Neurodegeneration. Front Neurol. 2019 Jul 31;10:807. doi: 10.3389/fneur.2019.00807.

> Poizat G, Alexandre C, Al Rifai S, Riffault L, Crepin D, Benomar Y, Taouis M. Maternal resistin predisposes offspring to hypothalamic inflammation and body weight gain. PLoS One. 2019 Mar 7;14(3):e0213267. doi: 10.1371/journal.pone.0213267. eCollection 2019. PMID: 30845245

> Campana M, Bellini L, Rouch C, Rachdi L, Coant N, Butin N, Bandet CL, Philippe E, Meneyrol K, Kassis N, Dairou J, Hajduch E, Colsch B, Magnan C, Le Stunff H. Inhibition of central de novo ceramide synthesis restores insulin signaling in hypothalamus and enhances β-cell function of obese Zucker rats. Mol Metab. 2018 Feb;8:23-36. doi: 10.1016.

> Benoit C, Doubi-Kadmiri S, Benigni X, Crepin D, Riffault L, Poizat G, Vacher CM, Taouis M, Baroin-Tourancheau A, Amar L. miRNA Long-Term Response to Early Metabolic Environmental Challenge in Hypothalamic Arcuate Nucleus. Front Mol Neurosci. 2018 Mar 28;11:90. doi: 10.3389/fnmol.2018.00090. eCollection 2018. PMID: 29643765

> Cruciani-Guglielmacci C, López M, Campana M, le Stunff H. Brain Ceramide Metabolism in the Control of Energy Balance. Front Physiol. 2017 Oct 12;8:787. doi:10.338. Review.PMID: 29075199

> Taouis M. MicroRNAs in the hypothalamus. Best Pract Res Clin Endocrinol Metab. 2016 Oct;30(5):641-651. doi: 10.1016/j.beem.2016.11.006. Epub 2016 Nov 15. Review.

Membres de l'équipe