TEFOR at Paris-Saclay

The TEFOR Paris-Saclay CNRS UMS2010 / INRAE UMS1451 (UMS TPS), a platform at the heart of the TEFOR infrastructure, serves researchers to conduct genome editing and phenotyping programs, particularly at early stages and in the adult brain of zebrafish.

• Genome editing: UMS TPS works with the plateforme TACGENE platform (U1154-UMR7196 CNRS/INSERM/MNHN) on "base editing" : targeted and fine editing of the genome on a single basis.

• Phenotyping : UMS TPS conducts programs to optimize microscopy tools and methodologies, and develops collections of optimal 3D image and navigation tools in these databases.

In order to offer a complete and innovative range of services, UMS TPS coordinates a network of expert centres: a consortium of laboratories and partner platforms whose complementary activities best meet the needs of the scientific community. The management of projects carried out for third party users is offered via a single point of contact, ensuring simplified monitoring of the user project, with as few administrative procedures as possible.

TEFOR infrastructure

The TEFOR infrastructure supports research on two alternative model species : zebrafish and drosophila. TEFOR is a distributed infrastructure composed
of ten partners : platforms and laboratories have partnered to provide innovative services in the fields of genome publishing, transgenesis, mutagenesis, and imaging phenotyping (EFOR).

It is funded by a “ Grand Emprunt Investissements d’Avenir ” ANR grant.

The major aims of the TEFOR infrastructure are :

• to provide transgenesis, mutagenesis, and phenotyping services and collaborative research projects in drosophila and zebrafish,
• to generate in both species new collections of transgenic lines,
• to develop technology projects in the areas of genome rewriting, viewing of 3D images and database archiving.

Recent publications :

> Mesencephalic origin of the inferior lobe in zebrafish.
Bloch S., Thomas M., Colin I., Galant S., Machado E., Affaticati P., Jenett A., Yamamoto K.
BMC Biol. 2019 Mar 8;17(1):22. Pubmed

> zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
Pierre Affaticati, Matthieu Simion, Elodie De Job, Laurie Rivière, Jean-Michel Hermel, Elodie Machado, Jean-Stéphane Joly, Arnim Jenett
Bio-Protocol 2017, Dec 5; 7;23:2636.

> Human amniotic fluid contaminants alter thyroid hormone signalling and early brain development in Xenopus embryos
Fini JB, Mughal BB, Le Mével S, Leemans M, Lettmann M, Spirhanzlova P, Affaticati P, Jenett A, Demeneix BA
Sci Rep. 2017 Mar 7;7:43786. Pubmed

> High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis.
Frétaud M, Rivière L, De Job É, Gay S, Lareyre JJ, Joly JS, Affaticati P, Thermes V
Sci Rep. 2017 Feb 17;7:43012. Pubmed

> Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes.
Passoni G, Langevin C, Palha N, Mounce BC, Briolat V, Affaticati P, De Job E, Joly JS, Vignuzzi M, Saleh MC, Herbomel P, Boudinot P, Levraud JP
Dis Model Mech. 2017 Jul 1;10(7):847-857. Pubmed

> Comparative analysis of monoaminergic cerebrospinal fluid-contacting cells in Osteichthyes (bony vertebrates).
Xavier AL, Fontaine R, Bloch S, Affaticati P, Jenett A, Demarque M, Vernier P, Yamamoto K
J Comp Neurol. 2017 Jun 15;525(9):2265-2283. Pubmed

> Postembryonic Fish Brain Proliferation Zones Exhibit Neuroepithelial-Type Gene Expression Profile.
Dambroise E, Simion M, Bourquard T, Bouffard S, Rizzi B, Jaszczyszyn Y, Bourge M, Affaticati P, Heuzé A, Jouralet J, Edouard J, Brown S, Thermes C, Poupon A, Reiter E, Sohm F, Bourrat Joly JS
Stem Cells. 2017 Jun;35(6):1505-1518. Pubmed

> A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth.
Mayrhofer M, Gourain V, Reischl M, Affaticati P, Jenett A, Joly JS, Benelli M, Demichelis F, Poliani PL,
Sieger D, Mione M

Dis Model Mech. 2017 Jan 1;10(1):15-28. Pubmed

TEFOR Paris-Saclay Website


Elodie Dejob & Pierre Affaticati