Julien Bouvier Team
Neuronal Circuits and Motor Control
Our research aims at identifying the neuronal circuits that execute movements and support their adaptive nature. We principally study breathing and locomotion, two vital behaviors that are largely conserved across species. Our current research projects aim at unveiling how breathing and locomotion are dynamically modulated according to internal or external needs, such as transition from rest to exercise, changes in posture or locomotor direction. These questions are addressed by a multidisciplinary strategy combining state-of-the-art viral-based anatomical tracings on dedicated transgenic mouse lines, neuro-anatomy, and functional investigations using electrophysiology, optogenetic and behavioral analysis.
To understand brain related diseases we need to understand the operation of the different neuronal networks that generate normal brain functions. This requires deciphering the identities and operating principles of cells executing of the function locally, as well as that of cells standing upstream its purposeful manifestation.
The team addresses this question on the motor behaviors of breathing and walking which are exquisite model systems with privileged experimental accessibility and strong translational outcomes. In these systems, tools from developmental biology are increasingly applied to manipulate – being to trace, record, kill, or modify the connectivity or activity of – subsets of neurons on the basis of their shared history of expression of specific developmental genes. This is progressively unlocking access to homogenous cell types embedded within complex architectures while informing on the intrinsic building logic of neural networks. In combination with functional investigations ex-vivo and behavior in vivo, this led rapid progress in identifying interneuronal subtypes with dedicated functions in the executive circuits for walking or breathing, often referred to as central pattern generators or CPGs. In contrast, the neuronal architecture that stand upstream the CPGs and condition their activity remains elusive. We are exploring this through two parallel projects that both touch upon the cooperative roles of clusters of brainstem and spinal cord neurons in elaborating adaptive respiratory and locomotor behaviors.
- Anatomical and functional investigation of the neuronal substrate linking respiration and locomotion. Respiratory increase during exercise is probably the most striking example of respiratory adaptation. While a direct influence of the locomotor neuronal circuit onto the respiratory CPG has been proposed, it has remained largely uncharted. We are currently exploring the neuronal connections that originate in the locomotor circuit, being the CPG in the spinal cord and/or its upstream controllers in the brainstem, and that impinge onto the respiratory CPG and upregulate breathing during locomotor engagement.
- Anatomical and functional investigation of reticulospinal neurons controlling locomotion. While the locomotor CPG contains all the elements necessary for producing the normal walking sequence, it is under obligatory control from descending signals conveyed by brainstem reticulospinal neurons. Yet the identities of reticulospinal neurons and that of their post-synaptic partners in the spinal CPG are still little resolved. We are currently addressing this with a particular focus on genetically-identified reticulospinal neurons that control limb movements.
Usseglio G, Gatier E, Heuzé A, Hérent C, Bouvier J. Control of orienting movements and locomotion by projection-defined subsets of brainstem V2a neurons. Current Biology, 2020. https://doi.org/10.1016/j.cub.2020.09.014. Open-access full text.
Skarlatou S, Hérent C, Toscano E, Mendes C, Bouvier J, Zampieri N. Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection. Cell Reports, 2020 Jun 9;31(10):107741. doi: 10.1016/j.celrep.2020.107741. Pubmed link. Open-access full text.
Caggiano V, Leiras R, Goñi-Erro H, Masini D, Bellardita C, Bouvier J, Caldeira V, Fisone G, Kiehn O. Midbrain circuits that set locomotor speed and gait selection. Nature. 2018 Jan 25;553(7689):455-460. Full text available on PMC. Abstract on Nature Website.
Bouvier J*#, Caggiano V*, Leiras R, Caldeira V, Bellardita C, Balueva K, Fuchs A, Kiehn O#. (*co-first and # co-corresponding). Descending Command Neurons in the Brainstem that Halt Locomotion. Cell. 2015 Nov 19;163(5):1191-1203. Full text available on Cell website.
Talpalar A*, Bouvier J*, Borgius L, Fortin G, Pierani A, Kiehn O. (*co-first authors). Dual mode operation of neuronal networks involved in left-right alternation. Nature. 2013 Aug 1;500(7460):85-8. Pubmed.