Mohammed Taouis Team

Molecular Neuroendocrinology of Food Intake

In brief

Our team studies the mechanisms involved in the onset of the hypothalamic leptin/insulin resistance. Our objective is to understand how inappropriate endocrine and metabolic environments during early life durably impair hypothalamic neuronal organization and leptin/insulin signaling.

Our research

Scientific projects
In line with our previous findings we will continue our focus on two main objectives :
1. Molecular and cellular mechanisms involved in hypothalamic insulin and leptin resistance
* Cellular and molecular mechanisms of resistin/TLR4-dependent hypothalamic nuclei inflammation and insulin resistance
Resistin induces hypothalamic neuro-inflammation (Benomar et al., 2013)

2. Impact of hypothalamic hormonal and metabolic environment during perinatal periods
* Expression profile of hypothalamic microRNA :
According to our previous studies we hypothesize that changes of specific miRNA expression levels may be involved, at least partially, in the process of diet or hormonal induced alteration of hypothalamic neuronal organization during early life. Furthermore, we will continue the improvement this technique by developing a guideline to quantify barcoding impact in multiplex microRNA expression profiles using Illumina technology.

Validation and the physiological relevance of miRNAs.
The characterization of specific miRNAs will be validated by in situ hybridization (ISH) to specifically confirm their localization in specific hypothalamic nuclei.

Validation and the physiological relevance of hypothalamic miRNAs.

Selected Publications

> Benomar Y, Taouis M. Molecular Mechanisms Underlying Obesity-Induced Hypothalamic Inflammation and Insulin Resistance: Pivotal Role of Resistin/TLR4 Pathways. Front Endocrinol (Lausanne). 2019 Mar 8;10:140. doi: 10.3389/fendo.2019.00140. eCollection 2019. Review.PMID: 30906281

> Le Stunff H, Véret J, Kassis N, Denom J, Meneyrol K, Paul JL, Cruciani-Guglielmacci C, Magnan C, Janel N. Deciphering the Link Between Hyperhomocysteinemia and Ceramide Metabolism in Alzheimer-Type Neurodegeneration. Front Neurol. 2019 Jul 31;10:807. doi: 10.3389/fneur.2019.00807.

> Poizat G, Alexandre C, Al Rifai S, Riffault L, Crepin D, Benomar Y, Taouis M. Maternal resistin predisposes offspring to hypothalamic inflammation and body weight gain. PLoS One. 2019 Mar 7;14(3):e0213267. doi: 10.1371/journal.pone.0213267. eCollection 2019. PMID: 30845245

> Campana M, Bellini L, Rouch C, Rachdi L, Coant N, Butin N, Bandet CL, Philippe E, Meneyrol K, Kassis N, Dairou J, Hajduch E, Colsch B, Magnan C, Le Stunff H. Inhibition of central de novo ceramide synthesis restores insulin signaling in hypothalamus and enhances β-cell function of obese Zucker rats. Mol Metab. 2018 Feb;8:23-36. doi: 10.1016.

> Benoit C, Doubi-Kadmiri S, Benigni X, Crepin D, Riffault L, Poizat G, Vacher CM, Taouis M, Baroin-Tourancheau A, Amar L. miRNA Long-Term Response to Early Metabolic Environmental Challenge in Hypothalamic Arcuate Nucleus. Front Mol Neurosci. 2018 Mar 28;11:90. doi: 10.3389/fnmol.2018.00090. eCollection 2018. PMID: 29643765

> Cruciani-Guglielmacci C, López M, Campana M, le Stunff H. Brain Ceramide Metabolism in the Control of Energy Balance. Front Physiol. 2017 Oct 12;8:787. doi:10.338. Review.PMID: 29075199

> Taouis M. MicroRNAs in the hypothalamus. Best Pract Res Clin Endocrinol Metab. 2016 Oct;30(5):641-651. doi: 10.1016/j.beem.2016.11.006. Epub 2016 Nov 15. Review.

Team members