Jean-René Martin Team

Functional Brain Imaging & Behavior

In brief

Aging, longevity, and neurodegenerative diseases are major concerns of public health. Our team is focalized on the characterization of the role of a new snoRNA (jouvence) that we have identified in Drosophila. Jouvence increases lifespan and protects against neurodegenerative lesions. It is required in the epithelium of the gut, revealing a new gut-brain axis. Since the snoRNAs are well conserved through evolution, we have identified its homologue in human, and we are currently characterizing its role in human culture cells. In parallel, using the in-vivo functional brain imaging approach that we have developed in our laboratory, based on bioluminescence, we study the role of the particular retrograde peak of Ca2+-activity in the Mushroom-Bodies, and more precisely its role in anaesthesia, whose represents a new model/system to study the consciousness.

Our research

The thematic of my team concerns the study of the neural bases of some behavior, as locomotor activity and more recently of olfaction, using a genetically tractable model system, the Drosophila. We use pluridisciplinary approaches, from genes characterization (molecular cloning) up to the whole integrated function, the locomotor activity and olfaction.
Methodologically, this implies the genetic and molecular biology, the neuroanatomy, the behavioral analysis and recently, the in-vivo functional brain imaging, by a bioluminescence approach. We have developed a video-tracking paradigm permitting to precisely quantifying the locomotor activity and allowing revealing its multiple complex parameters. More recently, we have develop a new in-vivo brain imaging approach, in bioluminescence, based on a chimeric protein (GFP-aequorin), allowing to record the neuronal activity.
Three axes are currently studied, in parallel.

Selected Publications

> Soulé S, Mellottée L, Arab A, Chen C, Martin JR. (2020). Jouvence a small nucleolar RNA required in the gut extends lifespan in Drosophila. Nat Commun 11, 987. https://doi.org/10.1038/s41467-020-14784-1

> Lark A, Kitamoto T, Martin JR. (2017). Modulation of neuronal activity in the Drosophila mushroom body by DopEcR, a unique dual receptor for ecdysone and dopamine. Biochim Biophys Acta, Mol. Cell Res., 1864, 1578-1588.

> Murmu MS, Martin, JR (2016). Interaction between cAMP and intracellular Ca2+-signaling pathways during odor-perception and adaptation in Drosophila. Biochim Biophys Acta, Mol. Cell Res., 1863, 2156-2174.

> Lark AR, Kitamoto T, Martin JR (2016). In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin. J Vis Exp., 107, doi: 10.3791/53705.

> Pierre Pavot, Elena Carbognin, Jean-René Martin (2015). PKA and cAMP/CNG Channels Independently Regulate the Cholinergic Ca2+-Response of Drosophila Mushroom Body Neurons. eNeuro, DOI: 10.1523/ENEURO.0054-14.2015

> Martin J.R, Rogers KL, Chagneau C, Brûlet P (2007) In vivo Bioluminescence Imaging of Ca2+ Signalling in the Brain of Drosophila. PLoS ONE 2(3): e275.

> Martin, J.-R. (2004). A portrait of locomotor behaviour in Drosophila determined by a video-tracking paradigm. Behav. Process., 67, 207-219.

> Martin, J.R., Faure, F., and Ernst, R. (2002). The Power Law Distribution for Walking-Time Intervals Correlates with the Ellipsoid Body in Drosophila. J. Neurogenetics, 15, 1-15.

Team members

Alumni

PostDocs
• Meena Murmu, Post-Doctorante (Avril 2007- Juillet 2009)
• Tsuyoshi Inoshita, Post-Doctorant (Juillet - Novembre 2007)
• Amélie Avet-Rochex (Post-Doctorante: avril - octobre 2006)
• Nicolas Meunier (Post-Doctorant: octobre 2003 - octobre 2004)

PhD
• Yesser Hadj Belgacem (D.E.A et Thèse) (Septembre 2002 - Décembre 2006)

D.E.A et Master (M2)
• Francisca Baggio (Master-2, Erasmus) (septembre 2007 - Juin 2008)
• Elena Carbognin (Master-2, Erasmus) (septembre 2006 - Juin 2007)
• Sébastien Lebreton (Master-2) (septembre 2005 - Juin 2006)
• Muriel Basile (Master-2) (septembre 2004 - septembre 2005)
• Lena Costille (D.E.A.) (septembre 2003 - septembre 2004)
• Morgane Besson (D.E.A.) (septembre 2001 - septembre 2002)
• Solange Kowalski (D.E.A.) (septembre 2001 - septembre 2002)
• Sylvain Gatti (D.E.A.) (septembre 1998 - septembre 1999)